Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus.
Identifieur interne : 001132 ( Main/Exploration ); précédent : 001131; suivant : 001133Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus.
Auteurs : Rosemary K. Le [États-Unis] ; Parthapratim Das [États-Unis] ; Kristina M. Mahan [États-Unis] ; Seth A. Anderson [États-Unis] ; Tyrone Wells [États-Unis] ; Joshua S. Yuan [États-Unis] ; Arthur J. Ragauskas [États-Unis]Source :
- AMB Express [ 2191-0855 ] ; 2017.
Abstract
Use of oleaginous microorganisms as "micro-factories" for accumulation of single cell oils for biofuel production has increased significantly to mitigate growing energy demands, resulting in efforts to upgrade industrial waste, such as second-generation lignocellulosic residues, into potential feedstocks. Dilute-acid pretreatment (DAP) is commonly used to alter the physicochemical properties of lignocellulosic materials and is typically coupled with simultaneous saccharification and fermentation (SSF) for conversion of sugars into ethanol. The resulting DAP residues are usually processed as a waste stream, e.g. burned for power, but this provides minimal value. Alternatively, these wastes can be utilized as feedstock to generate lipids, which can be converted to biofuel. DAP-SSF residues were generated from pine, poplar, and switchgrass. High performance liquid chromatography revealed less than 0.13% monomeric sugars in the dry residue. Fourier transform infrared spectroscopy was indicative of the presence of lignin and polysaccharides. Gel permeation chromatography suggested the bacterial strains preferred molecules with molecular weight ~ 400-500 g/mol. DAP-SSF residues were used as the sole carbon source for lipid production by Rhodococcus opacus DSM 1069 and PD630 in batch fermentations. Depending on the strain of Rhodococcus employed, 9-11 lipids for PD630 and DSM 1069 were observed, at a final concentration of ~ 15 mg/L fatty acid methyl esters (FAME) detected. Though the DAP-SSF substrate resulted in low FAME titers, novel analysis of solid-state fermentations was investigated, which determined that DAP-SSF residues could be a viable feedstock for lipid generation.
DOI: 10.1186/s13568-017-0484-0
PubMed: 28963644
PubMed Central: PMC5622019
Affiliations:
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Le</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Das, Parthapratim" sort="Das, Parthapratim" uniqKey="Das P" first="Parthapratim" last="Das">Parthapratim Das</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Mahan, Kristina M" sort="Mahan, Kristina M" uniqKey="Mahan K" first="Kristina M" last="Mahan">Kristina M. Mahan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Anderson, Seth A" sort="Anderson, Seth A" uniqKey="Anderson S" first="Seth A" last="Anderson">Seth A. Anderson</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Wells, Tyrone" sort="Wells, Tyrone" uniqKey="Wells T" first="Tyrone" last="Wells">Tyrone Wells</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, Joshua S" sort="Yuan, Joshua S" uniqKey="Yuan J" first="Joshua S" last="Yuan">Joshua S. Yuan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology & Microbiology, Texas A&M University, College Station, TX, 77843, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology & Microbiology, Texas A&M University, College Station, TX, 77843</wicri:regionArea><wicri:noRegion>77843</wicri:noRegion></affiliation></author><author><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA. aragausk@utk.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Center for Renewable Carbon, Department of Forestry, Wildlife, and Fisheries, University Tennessee Institute of Agriculture, Knoxville, TN, 37996, USA. aragausk@utk.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Renewable Carbon, Department of Forestry, Wildlife, and Fisheries, University Tennessee Institute of Agriculture, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Joint Institute for Biological Sciences, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA. aragausk@utk.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Joint Institute for Biological Sciences, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830</wicri:regionArea><wicri:noRegion>37830</wicri:noRegion></affiliation></author></analytic><series><title level="j">AMB Express</title><idno type="ISSN">2191-0855</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Use of oleaginous microorganisms as "micro-factories" for accumulation of single cell oils for biofuel production has increased significantly to mitigate growing energy demands, resulting in efforts to upgrade industrial waste, such as second-generation lignocellulosic residues, into potential feedstocks. Dilute-acid pretreatment (DAP) is commonly used to alter the physicochemical properties of lignocellulosic materials and is typically coupled with simultaneous saccharification and fermentation (SSF) for conversion of sugars into ethanol. The resulting DAP residues are usually processed as a waste stream, e.g. burned for power, but this provides minimal value. Alternatively, these wastes can be utilized as feedstock to generate lipids, which can be converted to biofuel. DAP-SSF residues were generated from pine, poplar, and switchgrass. High performance liquid chromatography revealed less than 0.13% monomeric sugars in the dry residue. Fourier transform infrared spectroscopy was indicative of the presence of lignin and polysaccharides. Gel permeation chromatography suggested the bacterial strains preferred molecules with molecular weight ~ 400-500 g/mol. DAP-SSF residues were used as the sole carbon source for lipid production by Rhodococcus opacus DSM 1069 and PD630 in batch fermentations. Depending on the strain of Rhodococcus employed, 9-11 lipids for PD630 and DSM 1069 were observed, at a final concentration of ~ 15 mg/L fatty acid methyl esters (FAME) detected. Though the DAP-SSF substrate resulted in low FAME titers, novel analysis of solid-state fermentations was investigated, which determined that DAP-SSF residues could be a viable feedstock for lipid generation.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">28963644</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2191-0855</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>1</Issue><PubDate><Year>2017</Year><Month>Sep</Month><Day>29</Day></PubDate></JournalIssue><Title>AMB Express</Title><ISOAbbreviation>AMB Express</ISOAbbreviation></Journal><ArticleTitle>Utilization of simultaneous saccharification and fermentation residues as feedstock for lipid accumulation in Rhodococcus opacus.</ArticleTitle><Pagination><MedlinePgn>185</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13568-017-0484-0</ELocationID><Abstract><AbstractText>Use of oleaginous microorganisms as "micro-factories" for accumulation of single cell oils for biofuel production has increased significantly to mitigate growing energy demands, resulting in efforts to upgrade industrial waste, such as second-generation lignocellulosic residues, into potential feedstocks. Dilute-acid pretreatment (DAP) is commonly used to alter the physicochemical properties of lignocellulosic materials and is typically coupled with simultaneous saccharification and fermentation (SSF) for conversion of sugars into ethanol. The resulting DAP residues are usually processed as a waste stream, e.g. burned for power, but this provides minimal value. Alternatively, these wastes can be utilized as feedstock to generate lipids, which can be converted to biofuel. DAP-SSF residues were generated from pine, poplar, and switchgrass. High performance liquid chromatography revealed less than 0.13% monomeric sugars in the dry residue. Fourier transform infrared spectroscopy was indicative of the presence of lignin and polysaccharides. Gel permeation chromatography suggested the bacterial strains preferred molecules with molecular weight ~ 400-500 g/mol. DAP-SSF residues were used as the sole carbon source for lipid production by Rhodococcus opacus DSM 1069 and PD630 in batch fermentations. Depending on the strain of Rhodococcus employed, 9-11 lipids for PD630 and DSM 1069 were observed, at a final concentration of ~ 15 mg/L fatty acid methyl esters (FAME) detected. Though the DAP-SSF substrate resulted in low FAME titers, novel analysis of solid-state fermentations was investigated, which determined that DAP-SSF residues could be a viable feedstock for lipid generation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Le</LastName><ForeName>Rosemary K</ForeName><Initials>RK</Initials><AffiliationInfo><Affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Das</LastName><ForeName>Parthapratim</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mahan</LastName><ForeName>Kristina M</ForeName><Initials>KM</Initials><AffiliationInfo><Affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Anderson</LastName><ForeName>Seth A</ForeName><Initials>SA</Initials><AffiliationInfo><Affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wells</LastName><ForeName>Tyrone</ForeName><Initials>T</Initials><Suffix>Jr</Suffix><AffiliationInfo><Affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Joshua S</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology & Microbiology, Texas A&M University, College Station, TX, 77843, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ragauskas</LastName><ForeName>Arthur J</ForeName><Initials>AJ</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-3536-554X</Identifier><AffiliationInfo><Affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN, 37996, USA. aragausk@utk.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Renewable Carbon, Department of Forestry, Wildlife, and Fisheries, University Tennessee Institute of Agriculture, Knoxville, TN, 37996, USA. aragausk@utk.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Joint Institute for Biological Sciences, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37830, USA. aragausk@utk.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>09</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>AMB Express</MedlineTA><NlmUniqueID>101561785</NlmUniqueID><ISSNLinking>2191-0855</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biofuel</Keyword><Keyword MajorTopicYN="N">Dilute-acid pretreatment</Keyword><Keyword MajorTopicYN="N">Lipids</Keyword><Keyword MajorTopicYN="N">Rhodococcus opacus</Keyword><Keyword MajorTopicYN="N">Simultaneous saccharification and fermentation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>08</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>09</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>10</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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